The control of viruses in water is critical to preventing the spread of infectious viral diseases. Many oxidants can inactivate viruses, and this study aims to systematically compare the disinfection effects of ozone (O), peroxymonosulfate (PMS), and hydrogen peroxide (HO) on MS2 coliphage. The effects of oxidant dose and contact time on disinfection were explored, as were the disinfection effects of three oxidizing agents in secondary effluent. The 4-log inactivation of MS2 coliphage required 0.05 mM O, 0.5 mM PMS, or 25 mM HO with a contact time of 30 min. All three oxidants achieved at least 4-log disinfection within 30 min, and O required only 0.5 min. In secondary effluent, all three oxidants also achieved 4-log inactivation of MS2 coliphage. Excitation-emission matrix (EEM) results indicate that all three oxidants removed dissolved organic matter synchronously and O oxidized dissolved organic matter more thoroughly while maintaining disinfection efficacy. Considering the criteria of oxidant dose, contact time, and disinfection efficacy in secondary effluent, O is the best choice for MS2 coliphage disinfection among the three oxidants.
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http://dx.doi.org/10.1039/d3em00527e | DOI Listing |
Sci Total Environ
December 2024
Centre for Water Resources Studies, Department of Civil & Resource Engineering, Dalhousie University, 1360 Barrington Street, Halifax, Nova Scotia B3H 4R2, Canada. Electronic address:
The COVID-19 pandemic has underscored the need for effective viral tracking in aqueous environments, particularly for non-enteric viruses. Despite advances in wastewater monitoring, surveillance of viruses in freshwater remains limited due to traditional sampling challenges. This study refines GAC-based passive sampling protocols by determining optimal extraction and elution methods for enhancing the recovery of viral nucleic acids in freshwater.
View Article and Find Full Text PDFAnal Bioanal Chem
January 2025
Department of Food Science, University of Massachusetts, Amherst, MA, USA.
Magnetic ionic liquids (MILs) have proven effective as capture reagents for foodborne bacterial pathogens; however, there are currently no published studies regarding their use with foodborne, non-enveloped viruses. In this study, a protocol was evaluated for capture and recovery of bacteriophage MS2, a human norovirus surrogate, and purified viral genomic single stranded RNA (ssRNA) from an aqueous suspension using MILs. Transition metal-based MILs showed similar capture and recovery efficiency for both targets.
View Article and Find Full Text PDFEnviron Res
January 2025
Guangdong Provincial Engineering Research Center for Urban Water Recycling and Environmental Safety, Key Laboratory of Microorganism Application and Risk Control of Shenzhen, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Pathogenic microorganisms pose a severe risk to the aquatic environment and human health. Membrane bioreactors (MBRs) have attracted much attention due to their simultaneous biological treatment and virus retention, but membrane fouling is the main obstacle. This study explored the effect of micro-granular activated carbon (μGAC) on bacteriophage MS2 removal efficiency and membrane fouling in a flat-plate MBR.
View Article and Find Full Text PDFJ Appl Microbiol
December 2024
Fraunhofer Institute for Process Engineering and Packaging, Department Retention of Food Quality, Giggenhauser Straße 35, 85354 Freising, Germany.
Aims: UV-C based air cleaners may reduce the transmission of infectious diseases. However, microbiological validation is necessary to quantify their efficiency. In this study, the stability of aerosolized bacteriophages for validation purposes was investigated in a test room, before a UV-C based air cleaner was exemplarily evaluated regarding the inactivation of airborne bacteriophages.
View Article and Find Full Text PDFMethods Mol Biol
November 2024
Aix Marseille Univ, CEA, CNRS, BIAM, UMR7265, SAVE (Signalisation pour l'Adaptation des Végétaux à leur Environnement), Saint-Paul, France.
Transcriptional reprogramming plays a key role in a variety of biological processes. Recent advances in RNA imaging techniques have allowed to visualize, in vivo, transcription-related mechanisms in different organisms. The MS2 system constitutes a robust method that has been used for over two decades to image multiple steps of a transcript's life cycle from "birth to death" with high spatiotemporal resolution in the animal field.
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