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Soybean AROGENATE DEHYDRATASES (GmADTs): involvement in the cytosolic isoflavonoid metabolon or trans-organelle continuity? | LitMetric

AI Article Synopsis

  • - Soybean produces isoflavonoids, which are specialized metabolites derived from phenylalanine (Phe) and play crucial roles in plant defense and nitrogen-fixing bacteria interactions.
  • - The study identifies a nine-member family of arogenate dehydratase (ADT) isoforms in soybean, crucial for Phe synthesis and isoflavonoid production.
  • - GmADTs exhibit dual substrate specificity and are differentially expressed, with all isoforms present in chloroplast stromules and interacting with isoflavonoid metabolon components, highlighting their importance in isoflavonoid biosynthesis.

Article Abstract

Soybean () produces a class of phenylalanine (Phe) derived specialized metabolites, isoflavonoids. Isoflavonoids are unique to legumes and are involved in defense responses , and they are also necessary for nodule formation with nitrogen-fixing bacteria. Since Phe is a precursor of isoflavonoids, it stands to reason that the synthesis of Phe is coordinated with isoflavonoid production. Two putative AROGENATE DEHYDRATASE (ADT) isoforms were previously co-purified with the soybean isoflavonoid metabolon anchor ISOFLAVONE SYNTHASE2 (GmIFS2), however the family had not been characterized. Here, we present the identification of the nine member family. We determined that the GmADTs share sequences required for enzymatic activity and allosteric regulation with other characterized plant ADTs. Furthermore, the GmADTs are differentially expressed, and multiple members have dual substrate specificity, also acting as PREPHENATE DEHYDRATASES. All GmADT isoforms were detected in the stromules of chloroplasts, and they all interact with GmIFS2 in the cytosol. In addition, GmADT12A interacts with multiple other isoflavonoid metabolon members. These data substantiate the involvement of GmADT isoforms in the isoflavonoid metabolon.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10845154PMC
http://dx.doi.org/10.3389/fpls.2024.1307489DOI Listing

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