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Background: Centrifuge-free processing methods support stool Xpert Ultra testing for childhood tuberculosis (TB), but there are limited data on their accuracy, acceptability and usability.
Methods: We conducted a prospective evaluation of stool Xpert Ultra in India, South Africa, and Uganda with three methods: Stool Processing Kit (SPK), Simple One-Step (SOS), and Optimized Sucrose Flotation (OSF). Children <15 years old with presumptive TB had respiratory specimen testing with Xpert Ultra and culture.
Two coacting shadow enhancers regulate twin of eyeless expression during early Drosophila development.
Genetics
November 2024
Biology Department, Clark University, 950 Main Street, Worcester, MA 01610, USA.
The Drosophila PAX6 homolog twin of eyeless (toy) sits at the pinnacle of the genetic pathway controlling eye development, the retinal determination network. Expression of toy in the embryo is first detectable at cellular blastoderm stage 5 in an anterior-dorsal band in the presumptive procephalic neuroectoderm, which gives rise to the primordia of the visual system and brain. Although several maternal and gap transcription factors that generate positional information in the embryo have been implicated in controlling toy, the regulation of toy expression in the early embryo is currently not well characterized.
View Article and Find Full Text PDFBlown spun fibers-based colorimetric sensor for detecting trace zinc in seminal fluid.
Biosens Bioelectron
February 2025
Institute of Chemistry, Federal University of Bahia (UFBA), 40170-280, Salvador, BA, Brazil. Electronic address:
Rapid and accurate analysis of zinc in seminal fluid is an important tool for male infertility diagnosis and forensic investigation of sexual assault. Among the traditional techniques employed for this purpose, colorimetric solid-state sensors offer a simple and efficient alternative for screening this analyte. Herein, we evaluated the feasibility of using solution blow spinning as a fast, simple, and cost-effective method to develop porous membranes that can be manufactured as low-cost colorimetric solid-state sensing materials.
View Article and Find Full Text PDFAn e-learning platform for clinical reasoning in cardiovascular diseases: a study reporting on learner and tutor satisfaction.
BMC Med Educ
September 2024
Faculty of Medicine, University of Medicine and Pharmacy "Carol Davila" Bucharest, Bucharest, 050474, Romania.
Background: Medical students gain essential skills through hospital training and internships, which complement their theoretical education. However, virtual patient platforms have been shown to effectively promote clinical reasoning and enhance learning outcomes. This study evaluates a web-based platform designed for learning clinical reasoning in cardiovascular diseases, detailing its functionalities and user satisfaction.
View Article and Find Full Text PDFEndoscopic band ligation: a simple, safe, and effective method for refractory diverticular bleeding (with video).
Gastrointest Endosc
January 2025
Department of Gastroenterology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
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