N6-methyladenosine (mA) and pseudouridine (Ψ) are the two most abundant modifications in mammalian messenger RNA, but the coordination of their biological functions remains poorly understood. We develop a machine learning-based nanopore direct RNA sequencing method (NanoSPA) that simultaneously analyzes mA and Ψ in the human transcriptome. Applying NanoSPA to polysome profiling, we reveal opposing transcriptomic co-occurrence of mA and Ψ and synergistic, hierarchical effects of mA and Ψ on the polysome.
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| http://dx.doi.org/10.1038/s41587-024-02135-0 | DOI Listing |
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