Facile Microwave-Assisted Hydrothermal Synthesis of Copper Oxide Nanoneedle Arrays for Practical Biomedical Applications.

Introduction: Copper oxide nanoneedle arrays (CuO NAs) have been widely used as antibacterial agents and in therapeutic applications because of their unique physicochemical features, low cytotoxicity, low cost, exceptional antibacterial action, and significant interest in biomedicine. Various analytical techniques were used to assess the related phase constitution, optical characteristics, elemental content, and surface morphology. The X-ray diffraction (XRD) patterns and field-emission scanning electron microscopy (FE-SEM) micrographs revealed that the CuO NAs had a monoclinic phase with a nanoneedle-like shape. Our findings may cover the progress of innovative and effective anti-bacterial capabilities based on CuO NAs, which have been shown to be effective against various pathogens, making them ideal options for fighting bacterial infections.  Objective: This research aimed to synthesize CuO NAs using microwave-solvothermal (MW-ST) technology, explore their effectiveness, and assess their biological activity.

Methods: The CuO NAs were synthesized using the MW-ST process, and their properties were assessed using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FE-SEM), energy dispersive analysis (EDS), field emission transmission microscopy (FE-TEM), and ultraviolet-visible (UV-Vis) techniques. The biocompatibility of CuO NAs was determined through hemolytic assays, and their bioactivities like antioxidant and anti-inflammatory assays were also determined.

Results: The CuO NAs were successfully developed, and various analytical tools were used to characterize and validate their morphology, size, crystallinity, and elemental compositions. It has been shown in in-vitro investigations that a strong anti-inflammatory impact is demonstrated by the inhibition of protein denaturation with low hemolytic potential. As a result, CuO NAs have the potential to be an excellent choice for anti-inflammatory solicitations.

Conclusion: CuO NAs were synthesized and characterized with various advanced techniques, revealing the formation of nanoneedles-like morphology. Based on the experimental findings, CuO NAs have the potential for anti-microbial, anti-oxidant, anti-inflammatory, and anti-hemolytic activities. However, additional in-vivo testing is essential to properly evaluate their efficiency and safety.