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Paneth cell ontogeny in term and preterm ovine models. | LitMetric

AI Article Synopsis

  • Paneth cells play a crucial role in intestinal health by protecting stem cells, influencing microbiome composition, and modulating immunity, but limited research has been done on their development in larger mammals like sheep.
  • This study investigated the development, quantity, and location of Paneth cells in fetal and newborn lambs across various gestational ages, finding a significant concentration in the ileum and lower density in the jejunum and colon.
  • Results revealed that transient asphyxia at birth notably reduced the number of Paneth cells, indicating that sheep could be valuable models for studying Paneth cell biology in early life.

Article Abstract

Introduction: Paneth cells are critically important to intestinal health, including protecting intestinal stem cells, shaping the intestinal microbiome, and regulating host immunity. Understanding Paneth cell biology in the immature intestine is often modeled in rodents with little information in larger mammals such as sheep. Previous studies have only established the distribution pattern of Paneth cells in healthy adult sheep. Our study aimed to examine the ontogeny, quantification, and localization of Paneth cells in fetal and newborn lambs at different gestational ages and with perinatal transient asphyxia. We hypothesized that ovine Paneth cell distribution at birth resembles the pattern seen in humans (highest concentrations in the ileum) and that ovine Paneth cell density is gestation-dependent.

Methods: Intestinal samples were obtained from 126-127 (preterm, with and without perinatal transient asphyxia) and 140-141 (term) days gestation sheep. Samples were quantified per crypt in at least 100 crypts per animal and confirmed as Paneth cells through in immunohistochemistry.

Results: Paneth cells had significantly higher density in the ileum compared to the jejunum and were absent in the colon.

Discussion: Exposure to perinatal transient asphyxia acutely decreased Paneth cell numbers. These novel data support the possibility of utilizing ovine models for understanding Paneth cell biology in the fetus and neonate.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10839032PMC
http://dx.doi.org/10.3389/fvets.2024.1275293DOI Listing

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