Developing a versatile tool for studying kinetics of Selenate-Se removal from aqueous solution using a chemostat bioreactor.

Understanding the impact of various parameters on the kinetics of dissolved selenium (Se) removal in bioreactors can be a challenging task, primarily due to the mass transfer limitations inherent in bioreactors employing attached growth configurations. This study successfully established a proof-of-concept for the efficient removal of Se from aqueous solutions using a chemostat bioreactor that relies solely on suspended growth. The research investigated the effect of selenate-Se feed concentrations under two distinct Se concentration conditions. One experiment was conducted at a considerably elevated concentration of 25 mg/L to impose stress on the system and evaluate its response. Another experiment replicated an environmentally relevant concentration of 1 mg/L, mirroring the typical Se concentrations in mine water. The bioreactor, featuring a working volume of 0.35 L, was operated as an anaerobic, fully mixed chemostat with hydraulic retention times (HRTs) ranging from 5 to 0.25 days. The outcomes revealed the chemostat's capacity to remove up to 25 mg/L of dissolved Se from water for all HRTs exceeding 1 day, under otherwise optimal conditions encompassing temperature, pH, and salinity. The research's significance lies in the development of a versatile tool designed to examine Se removal kinetics within a system devoid of mass transfer limitations. Furthermore, this study verified the ability of the bacterial consortium, obtained from a mine-influenced environment and enriched in the laboratory, to grow and sustain Se removal activities within a chemostat operating with HRTs as short as 1 day.