sp. nov., an endophytic actinomycete isolated from L. in Xinjiang, PR China.

An endophytic actinomycete designated TRM65318, was isolated from the root of L. Its taxonomic status was determined using a polyphasic approach. Comparative 16S rRNA gene sequence analysis indicated that strain TRM65318 is phylogenetically most closely related to XHU 5031 (98.15 %) and DSM 15700 (97.78 %). The peptidoglycan belonged to type A4α. The polar lipids were phosphatidylinositol, phosphatidylglycerol, diphosphatidylglycerol, two unknown lipids and three glycolipids. The predominant menaquinones were MK-9(H) and MK-9(H) and the whole-cell sugars contained glucose, mannose and galactose. Major fatty acids were anteiso-C, iso-C and C. Strain TRM65318 had a genome size of 5881012 bp with a genome G+C content of 71.79 mol%. The average nucleotide identity and DNA-DNA hybridization values between strain TRM65318 and the most closely related species were much lower than the thresholds commonly used to define species. At the same time, differences in phenotypic and genotypic data showed that strain TRM65318 could be clearly distinguished from XHU 5031. Therefore, it is concluded that strain TRM65318 represents a novel species of the genus of . The proposed name for this organism is sp. nov., with type strain TRM65318 (=CCTCC AA 2019057=LMG 31679).