AI Article Synopsis

  • Liver cancers show significant variability both between individuals and within tumors, complicating diagnosis and treatment development.
  • Desorption electrospray ionization mass spectrometry (DESI-MS) is a powerful imaging technique that allows for direct analysis of metabolites in liver cancer samples without altering them.
  • This chapter outlines a protocol for using DESI-MS on liver cancer specimens to identify potential new biomarkers by comparing cancerous tissues to normal liver tissue.

Article Abstract

Liver cancers are characterized by interindividual and intratumoral heterogeneity, which makes early diagnosis and the development of therapies challenging. Desorption electrospray ionization mass spectrometry (DESI-MS) imaging is a potent and sensitive MS ionization technique for direct, unaltered 2D and 3D imaging of metabolites in complex biological samples. Indeed, DESI gently desorbs and ionizes analyte molecules from the sample surface using an electrospray source of highly charged aqueous spray droplets in ambient conditions. DESI-MS imaging of biological samples allows untargeted analysis and characterization of metabolites in liver cancers to identify new biomarkers of malignancy. In this chapter, we described a detailed protocol using liver cancer samples collected and stored for histopathology examination, either as frozen or as formalin-fixed, paraffin-embedded specimens. Such hepatocellular carcinoma samples can be subjected to DESI-MS analyses, illustrating the capacity of spatially resolved metabolomics to distinguish malignant lesions from adjacent normal liver tissue.

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Source
http://dx.doi.org/10.1007/978-1-0716-3694-7_15DOI Listing

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