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Automated, Quantitative Capillary Western Blots to Analyze Host Cell Proteins in COVID-19 Vaccine Produced in Vero Cell Line.
Vaccines (Basel)
December 2024
Analytical Research & Development, Merck & Co., Inc., Rahway, NJ 07065, USA.
Background/objectives: Host cell protein (HCP) content is a major attribute for biological and vaccine products that must be extensively characterized prior to product licensure. Enzyme Linked Immunosorbent Assay (ELISA) and Mass Spectrometry (MS) are conventional methods for quantitative host cell protein analysis in biologic and vaccine products. Both techniques are usually very tedious, labor-intensive, and challenging to transfer to other laboratories.
View Article and Find Full Text PDFDesign and Study of Pulsed Eddy Current Sensor for Detecting Surface Defects in Small-Diameter Bars.
Sensors (Basel)
December 2024
College of Automation & College of Artificial Intelligence, Nanjing University of Posts and Telecommunications, Nanjing 210023, China.
The design and study of pulsed eddy current sensors for detecting surface defects in small-diameter rods are highly significant. Accurate detection and identification of surface defects in small-diameter rods may be attained by the ongoing optimization of sensor design and enhancement of detection technologies. This article presents the construction of a non-coaxial differential eddy current sensor (Tx-Rx sensor) and examines the detection of surface defects in a small diameter bar.
View Article and Find Full Text PDFDelayed Sowing Reduced Wilt by Altering Soil Temperature and Humidity to Enhance Beneficial Rhizosphere Bacteria of Sunflower.
Microorganisms
November 2024
College of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010019, China.
Sunflower Wilt (SVW) caused by is a significant threat to sunflower production in China. This soilborne disease is difficult to control. It has been observed that delayed sowing reduces the severity of SVW on different varieties and across various locations.
View Article and Find Full Text PDFThermal, Molecular Dynamics, and Mechanical Properties of Poly(Ethylene Furanoate)/Poly(ε-Caprolactone) Block Copolymers.
Molecules
December 2024
Laboratory of Chemistry and Technology of Polymers and Colors, Department of Chemistry, Aristotle University of Thessaloniki, GR-54124 Thessaloniki, Greece.
This study presents the synthesis and characterization of a series of multiblock copolymers, poly(ethylene 2,5-furandicarboxylate)-poly(ε-caprolactone) (PEF-PCL), created through a combination of the two-step melt polycondensation method and ring opening polymerization, as sustainable alternatives to fossil-based plastics. The structural confirmation of these block copolymers was achieved through Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), ensuring the successful integration of PEF and PCL segments. X-ray Photoelectron Spectroscopy (XPS) was employed for chemical bonding and quantitative analysis, providing insights into the distribution and compatibility of the copolymer components.
View Article and Find Full Text PDFAdvancements in Mass Spectrometry-Based Targeted Metabolomics and Lipidomics: Implications for Clinical Research.
Molecules
December 2024
Faculty of Pharmacy, Ton Duc Thang University, Ho Chi Minh City 700000, Vietnam.
Targeted metabolomics and lipidomics are increasingly utilized in clinical research, providing quantitative and comprehensive assessments of metabolic profiles that underlie physiological and pathological mechanisms. These approaches enable the identification of critical metabolites and metabolic alterations essential for accurate diagnosis and precision treatment. Mass spectrometry, in combination with various separation techniques, offers a highly sensitive and specific platform for implementing targeted metabolomics and lipidomics in clinical settings.
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