Background: The mechanisms for spinal cord stimulation (SCS) to alleviate chronic pain are only partially known. We aimed to elucidate the roles of adenosine A1 and A3 receptors (A1R, A3R) in the inhibition of spinal nociceptive transmission by SCS, and further explored whether 2'-deoxycoformycin (dCF), an inhibitor of adenosine deaminase, can potentiate SCS-induced analgesia.
Methods: We used RNAscope and immunoblotting to examine the distributions of adora1 and adora3 expression, and levels of A1R and A3R proteins in the spinal cord of rats after tibial-spared nerve injury (SNI-t). Electrophysiology recording was conducted to examine how adenosine receptor antagonists, virus-mediated adora3 knockdown, and dCF affect SCS-induced inhibition of C-fibre-evoked spinal local field potential (C-LFP).
Results: Adora1 was predominantly expressed in neurones, whereas adora3 is highly expressed in microglial cells in the rat spinal cord. Spinal application of antagonists (100 μl) of A1R (8-cyclopentyl-1,3-dipropylxanthine [DPCPX], 50 μM) and A3R (MRS1523, 200 nM) augmented C-LFP in SNI-t rats (DPCPX: 1.39 [0.18] vs vehicle: 0.98 [0.05], P=0.046; MRS1523: 1.21 [0.07] vs vehicle: 0.91 [0.03], P=0.002). Both drugs also blocked inhibition of C-LFP by SCS. Conversely, dCF (0.1 mM) enhanced SCS-induced C-LFP inhibition (dCF: 0.60 [0.04] vs vehicle: 0.85 [0.02], P<0.001). In the behaviour study, dCF (100 nmol 15 μl, intrathecal) also enhanced inhibition of mechanical hypersensitivity by SCS in SNI-t rats.
Conclusions: Spinal A1R and A3R signalling can exert tonic suppression and also contribute to SCS-induced inhibition of spinal nociceptive transmission after nerve injury. Inhibition of adenosine deaminase may represent a novel adjuvant pharmacotherapy to enhance SCS-induced analgesia.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10925891 | PMC |
http://dx.doi.org/10.1016/j.bja.2024.01.005 | DOI Listing |
Int Immunopharmacol
December 2024
Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong Jiangsu 226001, China. Electronic address:
The role of immune cells is crucial in nerve regeneration following spinal cord injury. Kif15, a member of the kinesin family, has been shown to enhance macrophage phagocytosis. This study investigates the impact of Kif15 deficiency on immune cells in zebrafish with spinal cord injury.
View Article and Find Full Text PDFJ Med Case Rep
December 2024
Department of Neurosurgery, The First Affiliated Hospital of Henan University of Science and Technology, 24 JingHua Road, Luoyang, 471000, Henan, China.
Background: Spinal schwannomas presenting with an intraspinal hematoma or subarachnoid hemorrhage are extremely rare, and patients often have severe spinal cord compression symptoms. However, the mechanism underlying the bleeding remains unclear.
Case Presentation: We present the case of a 53-year-old Chinese female diagnosed with a T12 schwannoma accompanied by an intratumoral hematoma.
BMC Surg
December 2024
Department of Spine Surgery, Tianjin Union Medical Center, Tianjin, 300121, China.
Background: The best open side for unilateral open-door laminoplasty (UODL) to treat inconsistent cervical ossification of the posterior longitudinal ligament (OPLL) needs to be identified.
Methods: Thirty-one individuals with inconsistent OPLL who underwent UODL between January 2016 and December 2018 were retrospectively divided into two groups: when the side of the open door was consistent with the side of the larger ossification occupancy area, patients were placed in the Consistent group; when the side of the open door was contralateral to the side of the larger ossification occupancy area, patients were placed in the Contralateral group. The following parameters were evaluated: neck disability index (NDI) score, Japanese Orthopaedic Association (JOA) score, visual analog scale (VAS) score, postoperative laminoplasty opening width and angle, and spinal cord diameter ratio.
Clin Neurol Neurosurg
December 2024
Case Western Reserve University School of Medicine, Cleveland, OH, United States; Department of Neurological Surgery, University Hospitals Cleveland Medical Center, Cleveland, OH, United States. Electronic address:
Background: Degenerative cervical myelopathy is one of the most common causes of spinal cord dysfunction. Cervical laminoplasty is an excellent surgical procedure that address the underlying pathology along with motion preservation with various advantages over other surgical options. While the advantages are intuitive and are being proven in multiple recent studies, concerns regarding failure still remains precluding wider utilization despite evidence to the contrary.
View Article and Find Full Text PDFHealth Place
December 2024
Center for Outcomes and Assessment Research, Kessler Foundation, 1199 Pleasant Valley Way, West Orange, NJ, 07052, USA; Department of Physical Medicine and Rehabilitation, Rutgers New Jersey Medical School, 183 South Orange Avenue- Suite F-1560, Newark, NJ, 07101, USA.
Exposure to nature is associated with better mental health in the general population, but prior evidence suggests that people living with severe, chronic mobility impairment from paralysis due to spinal cord injury (SCI) may not experience similar benefits. Since many people living with SCI use wheelchairs and other medical devices for mobility, further exploration of how people living with mobility disability experience greenspace is needed to achieve equity in access to all public places. We assessed experiences with accessing greenspace reported in a sample of people living with chronic SCI and the meanings they ascribe to these experiences for their health and quality of life.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!