Background: Hepatocellular carcinoma (HCC) represents the major type of primary liver cancers, which is a most prevalent malignancy, as well as seriously threatens people's life and health. MiR-128-2 was reported to be associated with several human cancers, and is participated in the pathogenesis and development of cancers. Our research was performed to explore the expression profile and diagnostic significance of miR-128-2 in HCC.
Methods: The serum miR-128-2 expression was measured in 130 HCC subjects and 118 healthy people using quantitative real-time polymerase chain reaction (qRT-PCR) assay. Chi-square (ÿ) test was utilized to estimate the relationship of miR-128-2 level with clinicopathological characteristic. In addition, the diagnostic accuracy of miR-128-2 in HCC was estimated via receiver operating characteristic (ROC) curve analysis.
Results: Compared with healthy individuals, the level of miR-128-2 was obviously up-regulated in HCC cases (P < 0.01). And its level was closely related to AFP level (P = 0.013), TNM stage (P = 0.009) and differentiation (P = 0.036). Nevertheless, there was no obvious relationship with age, gender, tumor size, HBV status, ALT or metastasis (all P > 0.05). According to the ROC curve, serum miR-128-2 had a high value in discriminating HCC cases from control individuals, with a AUC value of 0.880 combing with the sensitivity of 79.2% and the specificity of 83.9%.
Conclusion: The serum level of miR-128-2 in cases with HCC was obviously increased, which was related to the malignant progression of HCC. Due to the reasonable sensitivity and specificity, miR-128-2 might be as a new and effective marker for the diagnosis of HCC.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.prp.2024.155178 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The biological function of miR-128-2 in hepatocellular carcinoma and its molecular mechanism functioning.
Pathol Res Pract
February 2024
Department of Oncology, Dandong Cancer Hospital, Dandong 118000, Liaoning, China.
Background: Hepatocellular carcinoma (HCC) represents the major type of primary liver cancers, which is a most prevalent malignancy, as well as seriously threatens people's life and health. MiR-128-2 was reported to be associated with several human cancers, and is participated in the pathogenesis and development of cancers. Our research was performed to explore the expression profile and diagnostic significance of miR-128-2 in HCC.
View Article and Find Full Text PDFNatural antisense transcript of MYOG regulates development and regeneration in skeletal muscle by shielding the binding sites of MicroRNAs of MYOG mRNA 3'UTR.
Biochem Biophys Res Commun
June 2023
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong Province, China; Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affair, South China Agricultural University, Guangzhou, 510642, China. Electronic address:
Article Synopsis
- Natural antisense transcripts (NATs) are RNA molecules that play a key role in regulating biological processes, particularly in the growth and development of skeletal muscle by influencing sense transcripts.
- Analysis of transcriptome data indicates that NATs make up a significant portion of long non-coding RNAs (lncRNAs), with a strong correlation to myoblast differentiation and functions related to RNA synthesis, protein transport, and cell cycle regulation.
- The specific NAT called MYOG-NAT not only promotes myoblast differentiation but also stabilizes MYOG mRNA by competing with certain microRNAs, with its absence leading to muscle fiber atrophy and delayed muscle regeneration.
Identification of Novel CSF-Derived miRNAs in Treated Paediatric Onset Spinal Muscular Atrophy: An Exploratory Study.
Pharmaceutics
January 2023
Department of Neurology, Sydney Children's Hospital Network, Sydney, NSW 2031, Australia.
The availability of disease modifying therapies for spinal muscular atrophy (SMA) have created an urgent need to identify clinically meaningful biomarkers that provide insight into disease progression and therapeutic response. microRNAs (miRNA) have been shown to be involved in the pathogenesis of SMA and have the potential to provide insight within the field of SMA. miRNA-sequencing was utilized to identify differential miRNA expression in the cerebrospinal fluid (CSF) in six children with SMA treated with nusinersen in this exploratory study.
View Article and Find Full Text PDFProtective effects of the notoginsenoside R1 on acute lung injury by regulating the miR-128-2-5p/Tollip signaling pathway in rats with severe acute pancreatitis.
Innate Immun
January 2022
Department of Gastroenterology, First Affiliated Hospital of Dali University, Dali City, China.
Article Synopsis
- Notoginsenoside R1 (NG-R1) has shown potential as a treatment for acute lung injury (ALI) caused by severe acute pancreatitis (SAP) in rats, exhibiting anti-inflammatory effects.
- The study measured various gene and protein expression levels involved in inflammatory pathways, revealing that NG-R1 treatment lowers destabilizing factors like miR-128-2-5p while enhancing protective ones like Tollip.
- The mechanisms include reduced inflammatory responses and modulated signaling pathways (specifically HMGB1/TLR4 and ERK1), suggesting NG-R1 could be an effective therapy for managing SAP-induced ALI.
PERK Signaling Controls Myoblast Differentiation by Regulating MicroRNA Networks.
Front Cell Dev Biol
May 2021
MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, China.
The unfolded protein response (UPR) plays important roles in various cells that have a high demand for protein folding, which are involved in the process of cell differentiation and development. Here, we separately knocked down the three sensors of the UPR in myoblasts and found that PERK knockdown led to a marked transformation in myoblasts from a fusiform to a rounded morphology, which suggests that PERK is required for early myoblast differentiation. Interestingly, knocking down PERK induced reprogramming of C2C12 myoblasts into stem-like cells by altering the miRNA networks associated with differentiation and stemness maintenance, and the PERK-ATF4 signaling pathway transactivated muscle differentiation-associated miRNAs in the early stage of myoblast differentiation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!