Effect of ischemia-reperfusion injury on elafin levels in rat liver.

Ulus Travma Acil Cerrahi Derg

Department of General Surgery, University of Health Sciences, Antalya Training and Research Hospital, Antalya, Türkiye.

Published: February 2024

AI Article Synopsis

  • The study aimed to assess serum elafin levels and its impact on liver injury due to ischemia-reperfusion in rats, comparing multiple groups with varying reperfusion durations after 30 minutes of induced liver ischemia.
  • Results indicated a significant decrease in elafin levels as reperfusion time increased, with corresponding increases in ischemia-modified albumin (IMA) and total oxidant status (TOS), highlighting deteriorating liver function.
  • Histopathologically, a higher grade of liver injury was associated with lower elafin levels and higher levels of liver enzymes (AST, ALT), indicating a relationship between elafin levels and liver damage severity.

Article Abstract

Background: The aim of this study was to quantify serum levels of elafin, a serine protease inhibitor, and to assess its effects on histopathological and biochemical parameters in hepatic ischemia-reperfusion injury.

Methods: Forty female Wistar albino rats were divided into five groups: Group 1 served as the control group. Liver ischemia was induced for 30 minutes in the other four groups. An additional 1-hour, 2-hour, and 3-hour reperfusion was induced in Groups 3, 4, and 5, respectively. At the end of the experiment, intracardiac blood samples were obtained for biochemical examination, and tissue samples from the liver were taken for histopathological examination. Levels of elafin, ischemia-modified albumin (IMA), total antioxi-dant status (TAS), and total oxidant status (TOS) were also examined.

Results: Serum elafin levels decreased beginning from Group 2, with the lowest level reached in Group 5 (p<0.01). The IMA level was the lowest in the control group and the highest in Group 5 (p<0.01). TOS, aspartate aminotransferase (AST), and alanine amino-transferase (ALT) levels were lowest in the control group and highest in Group 5 (p<0.01). Group 5 had the highest IMA/albumin ratio, although no significant differences were found between these four groups. The lowest TAS level was found in the control group, but a stable and significant increase was not detected in the other groups. No significant differences were found between the groups in terms of alkaline phosphatase (ALP) and albumin levels. A negative correlation was observed between serum elafin levels and AST, ALT, and TOS levels (p<0.01). The number of Grade 1 histopathological results was found to be higher in the groups with reperfusion (Groups 3, 4, 5). In histopathological subgroup analysis, while the elafin level was lower in Grade 1 group, AST, ALT, and TOS levels were higher (p<0.01). Additionally, the IMA/albumin ratio was found to be higher in the Grade 1 group (p=0.02).

Conclusion: In hepatic ischemia-reperfusion injury, elafin levels decreased as the reperfusion time increased. As the reperfusion time increased, both hepatocyte damage and oxidant capacity increased, with a negative correlation observed between these findings and elafin levels. Therefore, elafin may play a protective role in hepatic ischemia-reperfusion injury and could assist clinicians in assessing liver injury.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10977509PMC
http://dx.doi.org/10.14744/tjtes.2024.32728DOI Listing

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