The article "Long non-coding RNA FAL1 regulated cell proliferation through Akt pathway via targeting PDK1 in esophageal cancer cells", by X.-S. Liang, Y. Sun, T. Liu, published in Eur Rev Med Pharmacol Sci 2018; 22 (16): 5214-5222-DOI: 10.26355/eurrev_201808_15719-PMID: 30178844 has been retracted by the authors for the following reasons. The authors asked to retract this manuscript as they identified some problems with the data reported, and the dilution ratio of the antibody during the Western blot experimental operation is incorrect. In this article, the dilution of antibodies is described as follows: anti-cyclin D 1 (dilution 1:500, Abcam, Cambridge, MA, USA), anti-MMP7 (dilution 1:500, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p21 (dilution 1:400, Cell Signaling Technology, Danvers, MA, USA), anti-p-Akt (dilution 1:500, Abcam, Cambridge, MA, USA), anti-Akt (dilution 1:500, Abcam, Cambridge, MA, USA), and anti-p-actin (dilution 1:500, Santa Cruz Biotechnology, Santa Cruz, CA, USA). Nonetheless, authors affirm they only used a dilution of 1:200 for all antibodies, undermining the Western blot experimental operation. Thus, the article's conclusions are considered invalid and unreliable. This article has been withdrawn. The Publisher apologizes for any inconvenience this may cause. https://www.europeanreview.org/article/15719.

Download full-text PDF

Source
http://dx.doi.org/10.26355/eurrev_202401_35041DOI Listing

Publication Analysis

Top Keywords

dilution 1500
20
santa cruz
16
1500 abcam
12
abcam cambridge
12
cambridge usa
12
dilution
9
non-coding rna
8
rna fal1
8
fal1 regulated
8
regulated cell
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!