COSA-1 is essential for accurate meiosis in . Two null mutants ( and ) have been notably studied. These null mutants exhibit severe meiotic defects, hindering the observation of the subtle or dynamic nature of COSA-1 function. To overcome these limitations, we developed a strain with inducible COSA-1 degradation using the Auxin-Inducible Degron (AID) system. This strain exhibits normal fertility and COSA-1::GFP foci. Auxin treatment successfully depletes COSA-1, resulting in a 96% decrease in progeny viability and 12 univalent chromosomes in diakinesis oocytes. This strain serves as a valuable tool for studying the dynamics of COSA-1.
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| http://dx.doi.org/10.17912/micropub.biology.001087 | DOI Listing |
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