AI Article Synopsis
- - A study in Guangdong Province, China, observed that 6.4% of carbapenem-resistant Pseudomonas aeruginosa clinical isolates were resistant to ceftazidime-avibactam (CZA).
- - Whole-genome sequencing of 16 CZA-resistant strains identified the presence of class B carbapenem resistance genes and highlighted the role of the PA1874 gene in this resistance.
- - The findings underscore the importance of monitoring CZA-resistant strains and indicate that class B resistance genes are crucial to understanding CZA resistance mechanisms.
Article Abstract
Objectives: Resistance against ceftazidime-avibactam (CZA) in carbapenem-resistant Pseudomonas aeruginosa (CRPA) is emerging. This study was aimed at detecting the prevalence and molecular characteristics of CZA-resistant CRPA clinical isolates in Guangdong Province, China.
Methods: The antimicrobial susceptibility profile of these strains was determined. A subset of 16 CZA-resistant CRPA isolates was analysed by whole-genome sequencing (WGS). Genetic surroundings of carbapenem resistance genes and pan-genome-wide association analysis were further studied.
Results: Of the 250 CRPA isolates, CZA resistance rate was 6.4% (16/250). The minimum inhibitory concentration (MIC) of CZA range was from 0.25 to >256 mg/L. MIC and MIC were 2/4 and 8/4 mg/L, respectively. Among the 16 CZA-resistant CRPA strains, 31.3% (5/16) of them carried class B carbapenem resistance genes, including bla, bla, and bla, located on IncP-2 megaplasmids or chromosomes, respectively. Pan-genome-wide association analysis of accessory genes for CZA-susceptible or -resistant CRPA isolates showed that PA1874, a hypothetical protein containing BapA prefix-like domain, was enriched in CZA-resistant group significantly.
Conclusions: Class B carbapenem resistance genes play important roles in CZA resistance. Meanwhile, the PA1874 gene may be a novel mechanism involving in CZA resistance. It is necessary to continually monitor CZA-resistant CRPA isolates.
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| http://dx.doi.org/10.1016/j.jgar.2024.01.014 | DOI Listing |
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