AI Article Synopsis
- * The researchers developed a pH-sensitive sensing platform using AuNCs@HDS, which can detect urease levels with a linear response range of 0.5-22.5 U/L and a low limit of detection of 0.19 U/L.
- * They also designed a portable hydrogel kit that integrates with smartphones for easy, cost-effective, and on-site urease monitoring without the need for complex instruments.
Article Abstract
Sensitive and convenient sensing of urease and its inhibitors is exceptionally urgent in clinical diagnosis and new drug development. In this study, the gold nanoclusters (AuNCs) and hydroxyl double salt (HDS) were composited by a simple confinement effect to prepare highly fluorescent AuNCs@HDS composites to monitor urease and its drug inhibitors. HDS was used as a matrix to confine AuNCs (AuNCs@HDS), facilitating the emission intensity of AuNCs. However, acidic conditions (low pH) can disrupt the structure of HDS to break the confinement effect, and quench the fluorescence of AuNCs. Therefore, a sensing platform for pH-related enzyme urease detection was constructed based on the sensitive response of AuNCs@HDS to pH. This sensing platform had a linear response range of 0.5-22.5 U/L and a low limit of detection (LOD) of 0.19 U/L for urease. Moreover, this sensing platform was also applied to monitor urease inhibitors and urease in human saliva samples. Additionally, a portable hydrogel kit combined with a smartphone was developed for urease detection to achieve portable, low-cost, instrument-free, and on-site monitoring of urease.
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| http://dx.doi.org/10.1016/j.talanta.2024.125725 | DOI Listing |
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