The birefringence in a dual-hole microstructured optical fiber is numerically calculated and characterized with an optical frequency domain reflectometry (OFDR) method. Due to the asymmetric dual air holes in the cross-section, the polarized 01 and 01 modes propagate with different group velocities and time delays. Through a polarized coherent OFDR system in experiment, the Fresnel reflection peaks for each mode are separated in the frequency domain with their corresponding beat frequency. Thus, the group birefringence -9.68×10 is calculated with a beat frequency difference of 50.03 Hz between the 01 and 01 modes at a 6.2 m fiber end, which is in good agreement with that of -9.54×10 from the theoretical simulation. Our demonstration provides an accurate and flexible method for group birefringence characterization in microstructured optical fibers with complex cross-sectional structures.
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