AI Article Synopsis
- - The study involved analyzing genetic differences (SNPs) to detect blood transfusions in athletes suspected of doping.
- - DNA was extracted from dried blood spots and assessed using a rapid real-time PCR technique.
- - This method can successfully identify transfusions even with just 1% of donor DNA, showing greater sensitivity compared to older testing methods.
Article Abstract
We performed genotyping analysis of human biallelic polymorphisms (single nucleotide polymorphisms) for the detection of homologous blood transfusion in sports doping. DNA was extracted from dried blood spots and quantified real-time fast PCR. The method was proven to allow the detection of transfusions up to a donor percentage of 1%, with a significant improvement in terms of sensitivity with respect to both the reference cytofluorimetric method and a previously proposed strategy based on the DNA STR-based strategy.
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| http://dx.doi.org/10.1002/dta.3650 | DOI Listing |
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