AI Article Synopsis
- - The study presents a new DNA nanocarrier designed from palindromic fragments, creating a simple 3D structural unit (PS-unit) that can efficiently assemble into a spherical architecture.
- - The nanocarrier features a unique size-change mechanism that allows it to load a significant amount of siRNA (1987 siPLK1s) and deliver it directly to cancer cells in response to specific stimuli.
- - Upon entering cancer cells, the nanocarrier releases nearly all of its siRNA in response to glutathione, effectively inhibiting PLK1 protein expression and significantly reducing tumor growth without causing systemic toxicity in mice.
Article Abstract
Construction of a simple, reconfigurable, and stimuli-responsive DNA nanocarrier remains a technical challenge. In this contribution, by designing three palindromic fragments, a simplest four-sticky end-contained 3D structural unit (PS-unit) made of two same DNA components is proposed. Via regulating the rotation angle of central longitudinal axis of PS-unit, the oriented assembly of one-component spherical architecture is accomplished with high efficiency. Introduction of an aptamer and sticky tail warehouse into one component creates a size-change-reversible targeted siRNA delivery nanovehicle. Volume swelling of 20 nm allows one carrier to load 1987 siPLK1s. Once entering cancer cells and responding to glutathione (GSH) stimuli, siPLK1s are almost 100% released and original size of nanovehicle is restored, inhibiting the expression of PLK1 protein and substantially suppressing tumor growth (superior to commercial transfection agents) in tumor-bearing mice without systemic toxicity.
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| http://dx.doi.org/10.1002/adhm.202303865 | DOI Listing |
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