Role of as a Tumor Suppressor in Cervical Cancer.

Background: The gene is silenced in various types of cancer, including cervical cancer; we recently demonstrated that the gene is also silenced in cervical cancer by hypermethylation of the CpG island in the gene promoter. This study aims to analyze whether could be a tumor suppressor in cervical cancer.

Methods: After ectopic expressing SLC5A8 in the HeLa cell line, we evaluated its effects on cell behavior both and by Confocal immunofluorescence, cell proliferation, migration assays, and xenograft transplants.

Results: Overexpression of SLC5A8 in the HeLa cell line decreased its proliferation by arresting cancer cells in the G1 phase and inhibiting cellular migration. Furthermore, we observed that pyruvate increased the SLC5A8 effect, inducing S-phase arrest and inhibiting the entry into mitosis. SLC5A8 decreased tumor growth in xenograft transplants, significantly reducing the volume and tumor weight at 35 days of analysis.

Conclusions: In summary, our results indicate that has a role as a tumor suppressor in cervical cancer.