Application of dsRNA for Fungi Disease Management Sclerotinia sclerotiorum and Botrytis cinerea.

Sclerotinia sclerotiorum and Botrytis cinerea are two closely related necrotrophic plant pathogenic fungi that have garnered significant attention due to their broad host range and persistent environmental presence. The use of double-stranded RNA (dsRNA) to inhibit specific genes has demonstrated promising potential in disease management. Various RNA interference (RNAi) strategies have been developed and applied in disease management, including the use of dsRNA to silence specific genes, as well as other RNA-based approaches. These strategies show promise in controlling fungal diseases and have the potential for broader application in plant protection. Here, we present a protocol that outlines techniques for preparing dsRNA and spraying it directly to control fungal diseases. This protocol offers a practical and effective solution for managing plant diseases and has the potential to be widely applied in disease management.