Detection of dsRNA with Fluorescence In Situ Hybridization (FISH).

Fluorescence in situ hybridization (FISH) is a powerful technique used for detecting and localizing specific nucleic acid sequences in cells or tissues. Double-stranded RNA (dsRNA) is a type of RNA with complementary strands, highly produced during the replication cycle of RNA viruses. dsRNA plays an essential role in many biological processes, including the activation of RNA silencing. Here, we present an overview of how FISH can be employed to detect and locate dsRNA. The detection and localization of dsRNA through FISH provide valuable insights into RNA-mediated processes and their roles in various biological phenomena.