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Structural insights into how Cas9 targets nucleosomes.
Nat Commun
December 2024
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
The CRISPR-associated endonuclease Cas9 derived from prokaryotes is used as a genome editing, which targets specific genomic loci by single guide RNAs (sgRNAs). The eukaryotes, the target of genome editing, store their genome DNA in chromatin, in which the nucleosome is a basic unit. Despite previous structural analyses focusing on Cas9 cleaving free DNA, structural insights into Cas9 targeting of DNA within nucleosomes are limited, leading to uncertainties in understanding how Cas9 operates in the eukaryotic genome.
View Article and Find Full Text PDFPatient-specific cutting guides allow 1° precision in asymmetric anterior closing-wedge osteotomy.
J Exp Orthop
January 2025
Department of Trauma and Orthopaedics, Institute for Locomotion, Sainte-Marguerite Hospital Aix-Marseille University Marseille France.
Purpose: Asymmetric anterior closing-wedge high tibial osteotomy (ACWHTO) allows correction of both excessive posterior tibial slope (PTS) and varus deformity. However, the complexity of this surgery requires a high degree of accuracy, which is less likely to be achieved with standard instrumentations. This study aimed to determine the accuracy of 3D patient-specific cutting guides (PSCGs) to provide the accurate planned correction in the frontal and sagittal planes.
View Article and Find Full Text PDFA five-finger mnemonic for teaching schoolchildren the theoretical components of adult basic life support: a modified reactive Delphi-guided development and memorability pilot test with schoolchildren.
BMC Med Educ
December 2024
Emergency Department, Maribor University Medical Centre, Maribor, Slovenia.
Background: A mnemonic is a cognitive aid frequently used in health-related education. The main goal of this study was to develop and test a 5-finger mnemonic for teaching schoolchildren the theoretical aspects of adult Basic Life Support (BLS) steps, a process rarely described in the context of instructing laypersons.
Methods: Experts from the European Resuscitation Council's Basic Life Support Science and Education Committee (ERC BLS SEC), specializing in teaching adult BLS, participated in the first phase of the pilot study.
Risk factors for methotrexate treatment failure in tubal ectopic pregnancy: a retrospective cohort study.
BMC Pregnancy Childbirth
December 2024
Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, National Clinical Research Center for Obstetric & Gynecologic Diseases, Beijing, People's Republic of China.
Background: Ectopic pregnancy (EP) accounts for approximately 2% of all pregnancies, with tubal ectopic pregnancies (TEPs) being the most common type. Methotrexate (MTX) is a noninvasive and effective medical management option for TEP, but failure rates range from 10 to 36%, posing challenges in clinical practice. Identifying risk factors for MTX treatment failure is crucial to improve patient outcomes and guide clinical decision-making.
View Article and Find Full Text PDFConservation Genetics and Breeding using Molecular Genetic Markers in Japanese Quail ().
Front Biosci (Schol Ed)
December 2024
School of Biosciences, University of Kent, CT2 7NJ Canterbury, Kent, UK.
Background: The Japanese quail () is a small migratory bird whose main habitats are located in East Asia, Russia, China, Japan, Korea, and India. The Japanese quail was first introduced into the Iraqi research sector in the early 1980s. This investigation aimed to identify the genetic divergence between the available genetic lines of the Japanese quail in Iraq as a first step to conducting further conservation and breeding, benefiting from studying the genetic diversity related to productivity, adaptation, and immune susceptibility.
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