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Impact of the unconventional extraction on antioxidants and antimicrobial characteristics of hibiscus and olive leaves extracts.
Heliyon
January 2025
Food Science Department, Faculty of Agriculture, Zagazig University, Zagazig, 44511, Egypt.
The antioxidant extracts considered a very important food additive which promoting the protection of lipid and prolong the shelf life of food products. The aim of this investigation was decrease the time of extraction of hibiscus leaves extract (HLE) and olive leaves extract (OLE) from 48 h to only 6 h without reducing efficiency of these extracts. HPLC assay, scavenging radical activity by DPPH˙ (IC), inhibition lipid peroxidation by both β-Carotene/Linoleic Acid Bleaching (βCB) and Thiobarbituric Acid Reactive Substances (TBARs) assays, antibacterial and antifungal activities measured for different concentrations of ethanolic extracts by conventional extraction (CE) and difference in pressure extraction (DPE) methods, and the results shown a considerable in mean difference ( < 0.
View Article and Find Full Text PDFAnticarcinogenic cationic peptides derived from tryptic hydrolysis of β-lactoglobulin.
Front Mol Biosci
January 2025
Department of Biochemistry, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
Introduction: This study investigated the tryptic hydrolysis of β-lactoglobulin (BLG) for 30, 60, 90, and 120 min at 1/200 E/S (enzyme/substrate ratio, w/w) to prepare potentially anticarcinogenic peptides.
Methods: The properties of hydrolysates were characterized, including degree of hydrolysis, free amino acids, SDS-PAGE, FTIR, and antioxidant activity employing DPPH-assay, β-carotene/linoleic acid, and FRAP assay.
Results: BLG tryptic hydrolysate produced after 60 min hydrolysis recorded the highest antioxidant activity, and LCMS analysis revealed 162 peptides of molecular masses ranging from 800 to 5671Da, most of them are of hydrophobic nature.
Metabolite Profiling and Association Analysis of Leaf Tipburn in Heat-Tolerant Bunching Onion Varieties.
Plants (Basel)
January 2025
Laboratory of Vegetable Crop Science, Division of Life Science, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan.
The bunching onion is an important leafy vegetable, prized for its distinctive flavor and color. It is consumed year-round in Japan, where a stable supply is essential. However, in recent years, the challenges posed by climate change and global warming have resulted in adverse effects on bunching onions, including stunted growth, discoloration, and the development of leaf tipburn, threatening both crop quality and yield.
View Article and Find Full Text PDFSpecific Nutrients Mediate the Association of Food Insecurity and Sleep Regularity Index (SRI) in U.S. Adults: NHANES 2011-2014.
Nutrients
January 2025
Translational Biobehavioral and Health Disparities Branch, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.
Background/objectives: Food-insecure individuals are at risk for poor health outcomes, including substandard sleep health. A possible association of food insecurity with sleep regularity has not been explored, and factors contributing to the relationship between food insecurity and sleep are not well understood. This cross-sectional study explored the relationship between food insecurity and sleep regularity and identified specific nutrients that mediated the association.
View Article and Find Full Text PDF[Construction and application of an inducible transcriptional regulatory tool from in ].
Sheng Wu Gong Cheng Xue Bao
January 2025
Key Laboratory of Medicinal Molecule Science and Pharmaceutical Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.
Transcriptional regulation based on transcription factors is an effective regulatory method widely used in microbial cell factories. Currently, few naturally transcriptional regulatory elements have been discovered from and applied. Moreover, the discovered elements cannot meet the demand for specific metabolic regulation of exogenous compounds due to the high background expression or narrow dynamic ranges.
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