Antimicrobial Activity of Extract Used for Potential Application in the Prevention and Treatment of Oral Diseases.

: This study evaluated the antimicrobial effect and cytotoxic potential of the natural extract against (), the causative agent of dental caries, which is a typical oral disease, and (), which causes oral candidiasis. : was shaken in 70% ethanol for 12 h to obtain samples through enrichment and lyophilization. Then, 1, 5, 10, 20, 30, and 40 mg/mL of the extract were applied to and to demonstrate its antimicrobial effect after 24 h. The stability of extract on the survival rate of human keratinocytes (HaCaT) was confirmed using water-soluble tetrazolium salt (WST-1) analysis. A one-way ANOVA determined the difference between each group. A Tukey HSD test was performed as a post-hoc analysis at the 0.05 significance level. : extract showed antimicrobial effects against and that increased in a concentration-dependent manner. However, high concentrations affected cell growth and lowered cell survival. The half maximal inhibitory concentration (IC value) of extract showed a survival rate of 53.04% at a concentration of 30 mg/mL, which was found to be applicable. : Thus, extract may be used as a natural material for the prevention and treatment of oral diseases. demonstrates optimal antimicrobial effects without affecting cell proliferation and growth at 30 mg/mL concentration.