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Characterizing adjuvants' effects at murine immunoglobulin repertoire level. | LitMetric

AI Article Synopsis

  • Generating high-quality sequencing data and characterizing the effects of adjuvants at the repertoire level is complex, prompting the development of an IgSeq pipeline for better data standardization and analysis.
  • The study investigated the effects of two adjuvants, CpG and Alum, on the Ig heavy chain repertoire using an ovalbumin (OVA) mouse model, focusing on various metrics like mutation, gene usage, and clonal diversity across different tissues and isotypes.
  • Results showed distinct Ig repertoire profiles in the spleen and bone marrow of non-immunized mice, with pronounced differences post-immunization, indicating that CpG and Alum influence the immune response differently based on tissue type and antibody isotype.

Article Abstract

Generating large-scale, high-fidelity sequencing data is challenging and, furthermore, not much has been done to characterize adjuvants' effects at the repertoire level. Thus, we introduced an IgSeq pipeline that standardized library prep protocols and data analysis functions for accurate repertoire profiling. We then studied systemically effects of CpG and Alum on the Ig heavy chain repertoire using the ovalbumin (OVA) murine model. Ig repertoires of different tissues (spleen and bone marrow) and isotypes (IgG and IgM) were examined and compared in IGHV mutation, gene usage, CDR3 length, clonal diversity, and clonal selection. We found Ig repertoires of different compartments exhibited distinguishable profiles at the non-immunized steady state, and distinctions became more pronounced upon adjuvanted immunizations. Notably, Alum and CpG effects exhibited different tissue- and isotype-preferences. The former led to increased diversity of abundant clones in bone marrow, and the latter promoted the selection of IgG clones in both tissues.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10805652PMC
http://dx.doi.org/10.1016/j.isci.2023.108749DOI Listing

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