Background: Vancomycin resistance encoded by the // genes in enterococci is clinically important because of the transmission of these genes between bacteria. While vancomycin resistance is determined by detecting only and genes by routine analyses, failure to detect resistance causes vancomycin resistance to be overlooked, and clinically appropriate treatment cannot be provided.
Aims: The study aimed to examine the presence of -positive enterococcal isolates in Ankara, Turkey, and to have detailed information about them with sequence analyses.
Methods: Caecal samples were collected from sheep and cattle during slaughter at different slaughterhouses in Ankara, Turkey. Enterococci isolates were identified, confirmed, and analyzed for the presence of // genes. Antibiotic resistance profiles of isolates were determined by the broth microdilution method. A whole genome sequence analysis of the isolates harboring the and genes was performed.
Results: 13.7% of enterococcal isolates were determined as and . 15% of these isolates contained , and 40% were -positive. S98b and C32 isolates were determined to contain 16 CRISPR-Cas elements. 80% of the enterococci isolates were resistant to nitrofurantoin and 15% to ciprofloxacin. The first -positive vancomycin-variable enterococci (VVE) isolates from food-producing animals were identified, and the S98b strain has been assigned to Genbank with the accession number CP104083.1.
Conclusion: Therefore, new studies are needed to facilitate the identification of -resistant enterococci and VVE strains.
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| http://dx.doi.org/10.22099/IJVR.2023.47465.6855 | DOI Listing |
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