AI Article Synopsis
- Pioneer transcription factors, like PU.1 and C/EBPα, play a key role in changing cell types by regulating hematopoietic stem cell differentiation.
- The study reveals that PU.1 and C/EBPα work together to interact with nucleosomes, shifting DNA and unwrapping chromatin, which is crucial for gene accessibility.
- A mutation in PU.1 linked to leukemia disrupts its ability to interact with nucleosomes, highlighting the importance of these interactions in maintaining normal cell function.
Article Abstract
Pioneer transcription factors are vital for cell fate changes. PU.1 and C/EBPα work together to regulate hematopoietic stem cell differentiation. However, how they recognize in vivo nucleosomal DNA targets remains elusive. Here we report the structures of the nucleosome containing the mouse genomic CX3CR1 enhancer DNA and its complexes with PU.1 alone and with both PU.1 and the C/EBPα DNA binding domain. Our structures reveal that PU.1 binds the DNA motif at the exit linker, shifting 17 bp of DNA into the core region through interactions with H2A, unwrapping ~20 bp of nucleosomal DNA. C/EBPα binding, aided by PU.1's repositioning, unwraps ~25 bp of entry DNA. The PU.1 Q218H mutation, linked to acute myeloid leukemia, disrupts PU.1-H2A interactions. PU.1 and C/EBPα jointly displace linker histone H1 and open the H1-condensed nucleosome array. Our study unveils how two pioneer factors can work cooperatively to open closed chromatin by altering DNA positioning in the nucleosome.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1038/s41594-023-01189-z | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
High modulus and strength polyurethane film synthesized from lignin-based polyol with various lignin contents and NCO/OH molar ratios.
Int J Biol Macromol
January 2025
State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China. Electronic address:
This study explored the synthesis of lignin-polyol (LP) by liquefaction of Kraft lignin in polyethylene glycol and glycerol. LP23 (23 wt% lignin) and LP41 (41 wt% lignin) were obtained with remarkable liquefaction yields of 97.43 % and 93.
View Article and Find Full Text PDFExpression of microRNA-155 in thalassemic erythropoiesis.
PeerJ
September 2024
Faculty of Medical Technology, Prince of Songkla University, Hat Yai, Songkhla, Thailand.
Article Synopsis
- Ineffective erythropoiesis (IE) leads to anemia in beta-thalassemia, characterized by increased erythroblast proliferation without proper differentiation, resulting in immature red blood cells (RBCs).
- The study investigates the role of microRNA-155 (miR-155) in beta-thalassemia, focusing on its expression in erythroid cells from thalassemic bone marrow and its effects on cell differentiation and proliferation.
- Results show that miR-155 is upregulated during erythropoiesis in thalassemic mice, and manipulating its expression alters erythroid cell proliferation and differentiation, suggesting its potential significance in managing thalassemia-related anemia.
Fracture resistance of partial and complete coverage veneers and ceramic crowns for maxillary central incisors.
J Prosthet Dent
August 2024
Professor, Department of Prosthodontics, Indiana University School of Dentistry, Indianapolis, Ind; and Director, Center for Implant, Esthetic and Innovative Dentistry, Indiana University School of Dentistry, Indianapolis, Ind.
Statement Of Problem: Minimally invasive treatments have gained popularity in recent years. However, research comparing the fracture resistance of lithium disilicate partial coverage veneer restorations with that of ceramic crowns is lacking.
Purpose: The purpose of this in vitro study was to evaluate and compare the fracture resistance of lithium disilicate restorations fabricated for preparations of various designs.
Development of lactic acid based chain extender and soybean oil-derived polyurethanes for ecofriendly sustained drug delivery systems.
Int J Biol Macromol
April 2024
Department of Applied Sciences, National Textile University, Faisalabad-37610, Pakistan.
In the present study, a range of sustainable, biocompatible and biodegradable polyurethanes (PU-1 to PU-4) were synthesized using different combinations of biobased polyol (obtained through the epoxidation of soybean oil, followed by ring opening with ethanol) and polyethylene glycol (PEG) and isophorone diisocyanate. The sustainable chain extender used in this study was synthesized by the esterification of lactic acid with ethylene glycol (EG). The synthesized PU samples were characterized through scanning electron microscopy (SEM), Fourier transformed infrared (FTIR) and nuclear magnetic resonance (H NMR and C NMR) spectroscopy.
View Article and Find Full Text PDFSynthesis and Biodegradation Test of a New Polyether Polyurethane Foam Produced from PEG 400, -Lysine Ethyl Ester Diisocyanate (L-LDI) and Bis-hydroxymethyl Furan (BHMF).
Toxics
August 2023
Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Pedro Escobedo 76703, Mexico.
In this paper we produced a bio-based polyether-polyurethane foam PU1 through the prepolymer method. The prepolymer was obtained by the reaction of PEG 400 with -Lysine ethyl ester diisocyanate (L-LDI). The freshly prepared prepolymer was extended with 2,5-bis(hydroxymethyl)furan (BHMF) to produce the final polyurethane.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!