AI Article Synopsis
- Recent advances in microscopy technology have enhanced our ability to study how hosts interact with pathogens, particularly through live-cell imaging.
- Enhanced microscope hardware and engineered biosensors enable better visualization of live cells, allowing for real-time observation of signaling events and protein positioning.
- The text outlines a specific protocol for conducting long-term live-cell fluorescence imaging in virus-infected cell lines.
Article Abstract
Recent technological advances in microscopy have facilitated novel approaches to investigate host-pathogen interactions. In particular, improvements in both microscope hardware and engineered biosensors have helped to overcome barriers to live-cell imaging with fluorescence microscopy. Live fluorescent microscopy allows for the detection of discrete signaling events and protein localization, improving our ability to assess the effects of pharmacologic agents, microbes, or infection with high temporal resolution. Here we describe a protocol for long-term live-cell fluorescence imaging of virus infected cell lines.
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| http://dx.doi.org/10.1007/978-1-0716-3617-6_3 | DOI Listing |
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