Bacteria causing hospital-associated infections continue to become resistant due to antibiotic resistance, which has become a global problem worldwide and accordingly, the antibiotic options used in the treatment of infectious diseases caused by these bacteria are limited. In the light of the data obtained from experimental studies on plants, it is thought that plant extracts may be a promising option in the treatment of infectious diseases. In this study, it was aimed to determine the antibacterial activity of Taraxacum officinale extracts on Bacteroides fragilis ATCC 25285 standard strain by broth microdilution method and to pioneer different studies that will investigate the antibacterial effects of plant extracts on resistant B.fragilis strains that cause hospital-acquired opportunistic infections after invasive interventions and trauma. In this study, the T.officinale plant collected as a result of field work was divided into root, leaf and flower parts and dried at 70 °C for 24 hours and then turned into powder. Dried plant samples were extracted in ethanol and methanol for 24 hours. The obtained extracts were stored at -80 °C to be used in the broth microdilution method. B.fragilis ATCC 25285 standard strain was used as the bacterial strain. As a result of the experiments performed with broth microdilution method, the MIC (minimum inhibitory concentration) value of root, leaf and flower extracts with ethanol was determined as 200 μg/ mL, the methanolic root extract as 100 μg/mL and the methanolic leaf and flower extracts as 200 μg/mL. As a result, ethanol and methanol plant extracts were found to be effective on B.fragilis strain.
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http://dx.doi.org/10.5578/mb.20249906 | DOI Listing |
Front Cell Infect Microbiol
December 2024
Key Laboratory of Respiratory Disease, People's Hospital of Yangjiang, Yangjiang, China.
Ceftazidime-avibactam (CZA) is one of the effective antibiotics used for the treatment of carbapenem-resistant (CRKP) infections, but its resistance rate has increased recently. Previous studies have focused on the mechanisms of CZA resistance, while its heteroresistance in CRKP remains poorly understood. This study aimed to investigate the characteristics and mechanisms of CZA heteroresistance in CRKP isolates.
View Article and Find Full Text PDFRev Argent Microbiol
December 2024
Departamento de Bioquímica Clínica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Hospital de Clínicas «José de San Martín», Buenos Aires, Argentina. Electronic address:
Aeromonas spp. are opportunistic pathogens that cause both intra- and extraintestinal infections. The objective of this work was the phenotypic and genotypic characterization of a collection of Aeromonas strains, in addition to determining their sensitivity to different antimicrobials.
View Article and Find Full Text PDFInt J Antimicrob Agents
December 2024
Department of Respiratory and Critical Care Medicine, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200433, China; School of Medicine, Tongji University, Shanghai 200092, China; Shanghai Key Laboratory of Tuberculosis, Shanghai Pulmonary Hospital, School of Medicine, Tongji University, Shanghai 200433, China. Electronic address:
Background: β-lactams are crucial for anti-Mycobacterium abscessus complex (MABC) therapy. Treating infections is challenging since MABC produces a class A β-lactamase (Bla , which is capable of hydrolyzing β-lactams thus causing drug resistance. Diazabicyclooctane (DBO) β-lactamase inhibitors (BLIs) can inhibit Bla.
View Article and Find Full Text PDFMicrob Pathog
December 2024
Universidade Regional do Cariri - URCA, Rua Cel. Antônio Luiz, 1161 - Pimenta, Crato- CE.
Candidiasis is an infectious disease caused by some fungi of the genus Candida. In Brazil, the incidence rate is higher than in European countries and the United States, and health problems occur mainly due to the virulence factors of the fungi, which have made treatment with commercial drugs difficult. Considering the context, plants rich in phenolic compounds, such as those of the genus Piper, have been studied due to their antimicrobial properties.
View Article and Find Full Text PDFJ Microbiol Methods
December 2024
Medical Microbiology, Division of Infection and Immunity, Cardiff University, Cardiff, UK; UK Health Security Agency, Bacteriology Reference Department, London, UK. Electronic address:
Currently there is no detailed, internationally agreed protocol defined to evaluate antimicrobial susceptibility testing (AST) for Legionella pneumophila (required to establish epidemiological cut-off value or "ECOFF" boundaries); therefore, antimicrobial resistance in these isolates cannot be defined. AST methods utilising media containing activated charcoal as an ingredient, to enable Legionella growth, are unreliable as noted in an internationally authored opinion paper and a new gold standard is required. Here we define a detailed protocol for broth microdilution (BMD) using defined cell culture collection-deposited control reference strains (Philadelphia-1 and Knoxville-1) as well as two accessible reference strains with moderately (lpeAB-carrying) and markedly (23S rRNA mutation-carrying) elevated azithromycin minimum inhibitory concentration (MIC).
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