AI Article Synopsis
- Oral submucous fibrosis (OSF) is a serious oral condition that can turn cancerous, and this study focuses on how epithelial-to-mesenchymal transition (EMT) contributes to this risk by analyzing specific markers.
- The research involved examining tissue samples from different stages: OSF, oral squamous cell carcinoma related to OSF (OSFSCC), and general oral squamous cell carcinoma (OSCC), using immunohistochemical methods and transcriptomic analysis.
- Results showed that markers associated with epithelial characteristics decreased while those linked to mesenchymal properties increased in OSFSCC and OSCC, suggesting EMT plays a key role in OSF's progression toward cancer, particularly
Article Abstract
Background: Oral submucous fibrosis (OSF) is a persistent oral mucosal condition that carries an elevated risk of undergoing malignant transformation. Our objective was to elucidate the involvement of epithelial-to-mesenchymal transition (EMT) in OSF and its progression to malignancy by studying a panel of EMT markers, thereby understanding the molecular mechanisms.
Methods: An immunohistochemical analysis was done to detect the presence of E-cadherin, N-cadherin, pan-cytokeratin (PanCK), vimentin, α-SMA (alpha-smooth muscle actin), and CD44 in a total of 100 tissue samples. These samples comprised 40 cases of OSF, 20 cases of oral squamous cell carcinoma associated with OSF (OSFSCC), and 40 cases of oral squamous cell carcinoma (OSCC). A whole transcriptomic analysis was performed on a group of seven matched samples encompassing NOM, OSF, OSFSCC, and OSCC.
Results: We observed significantly decreased expression of E-cadherin and PanCK, while N-cadherin, vimentin, α-SMA, and CD44 showed significantly higher expression in OSFSCC and OSCC as compared to OSF, both at protein and RNA levels. CD44 expression was noticeably higher in OSFSCC (p < 0.001) than in OSCC.
Conclusion: Downregulation of epithelial markers with concomitant upregulation of mesenchymal and stem cell markers suggests the potential role of EMT and stemness in accelerating the pathogenesis and malignant transformation of OSF. The high levels of CD44 expression seen in OSFSCC indicate a high propensity for aggressiveness and acquisition of stem-like characteristics by the cells undergoing EMT.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10794927 | PMC |
| http://dx.doi.org/10.1016/j.jobcr.2023.12.006 | DOI Listing |
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