AI Article Synopsis
- The CRISPR-Cas9 system is a powerful genome-editing tool that can help address various genetic disorders by precisely targeting and modifying DNA.
- It consists of a single-guide RNA (sgRNA) that directs the Cas9 protein to specific DNA sequences, allowing for targeted editing.
- A major concern with CRISPR-Cas9 is off-target effects, where unintended DNA modifications occur, which can undermine its effectiveness and reliability; this review discusses recent improvements aimed at minimizing these off-target effects.
Article Abstract
The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)) and the associated protein (Cas9) system, a young but well-studied genome-editing tool, holds plausible solutions to a wide range of genetic disorders. The single-guide RNA (sgRNA) with a 20-base user-defined spacer sequence and the Cas9 endonuclease form the core of the CRISPR-Cas9 system. This sgRNA can direct the Cas9 nuclease to any genomic region that includes a protospacer adjacent motif (PAM) just downstream and matches the spacer sequence. The current challenge in the clinical applications of CRISPR-Cas9 genome-editing technology is the potential off-target effects that can cause DNA cleavage at the incorrect sites. Off-target genome editing confuses and diminishes the therapeutic potential of CRISPR-Cas9 in addition to potentially casting doubt on scientific findings regarding the activities of genes. In this review, we summarize the recent technological advancements in reducing the off-target effect of CRISPR-Cas9 genome editing.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10802171 | PMC |
| http://dx.doi.org/10.2147/BTT.S429411 | DOI Listing |
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