AI Article Synopsis
- The rising spread of arboviruses and increasing infection rates emphasize the urgent need for improved methods to rapidly and accurately detect these pathogens.
- This study focuses on combining nanopore sequencing with reverse transcription polymerase chain reaction (RT-PCR) to create a multiplex RT-PCR protocol.
- The new protocol allows the simultaneous detection of multiple viruses, including Chikungunya, dengue, Zika, yellow fever, and West Nile, enhancing our ability to differentiate between arbovirus strains effectively.
Article Abstract
The emergence and continued geographic expansion of arboviruses and the growing number of infected people have highlighted the need to develop and improve multiplex methods for rapid and specific detection of pathogens. Sequencing technologies are promising tools that can help in the laboratory diagnosis of conditions that share common symptoms, such as pathologies caused by emerging arboviruses. In this study, we integrated nanopore sequencing and the advantages of reverse transcription polymerase chain reaction (RT-PCR) to develop a multiplex RT-PCR protocol for the detection of Chikungunya virus (CHIKV) and several orthoflaviviruses (such as dengue (), Zika (), yellow fever (), and West Nile () viruses) in a single reaction, which provides data for sequence-based differentiation of arbovirus lineages.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10821003 | PMC |
| http://dx.doi.org/10.3390/v16010023 | DOI Listing |
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