Isolation and Structure Elucidation of New Metabolites from the Mariana-Trench-Associated Fungus sp. SY2601.

Fungi are important resource for the discovery of novel bioactive natural products. This study investigated the metabolites produced by Mariana-Trench-associated fungus sp. SY2601 in EY liquid and rice solid media, resulting in the isolation and structure determination of 28 metabolites, including five new compounds, asperindopiperazines A-C (-), 5-methoxy-8,9-dihydroxy-8,9-deoxyaspyrone (), and 12-aspertetranone D (). Structures of the new compounds were elucidated based on extensive NMR spectral analyses, HRESIMS data, optical rotation, ECD, and C NMR calculations. The new compound 12-aspertetranone D () exhibited antibacterial activity against both methicillin-resistant and with MIC values of 3.75 and 5 μg/mL, respectively.