AI Article Synopsis
- * Active compounds such as hydroxytyrosol and oleuropein were tested at varying concentrations, revealing that while 50 µM inhibited cell growth, it didn’t increase cell death, whereas 100 µM did both.
- * The findings confirm that these olive oil extracts have antiproliferative and pro-apoptotic properties, making them significant for further research in cancer and kidney disease protection.
Article Abstract
Background: Extra virgin olive oil is a typical food of the Mediterranean area, obtained by pressing L. fruits. Its polyphenols have been studied for their antioxidant function and protective action against cancer and chronic kidney disease. In this study, we tested titrated extracts from L. on a human embryonic kidney 293 (HEK-293E) cell line, regarding their pro-apoptotic and antiproliferative capacities, using " IncuCyte S3 Live-Cell Analysis System".
Materials And Methods: We selected L. active compounds like hydroxytyrosol (HT) and oleuropein (OLE). These extracts were tested at different concentrations and characterized by HPLC-DAD-MS for the content in secondary active metabolites. The real-time observation of cell behavior was performed by IncuCyte, which can quantitatively analyze the cell proliferation and death.
Results: This study showed that all the tested extracts can significantly inhibit cellular growth at 50 µM but the reduced proliferation is not related to an increase in cellular apoptosis. Instead, the same analysis performed by using extracts at 100 µM reveals that they can inhibit cellular growth, further inducing cellular apoptosis.
Conclusions: The results on the HEK-293E cells confirmed the antiproliferative and proapoptotic actions of active compounds from an L. matrix in this cell line.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10820526 | PMC |
| http://dx.doi.org/10.3390/life14010049 | DOI Listing |
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