506 Protects the Intestinal Barrier from the Damaging Effects of Enteric Pathogens and Deoxynivalenol.

This study investigated the impact of 506 on gut barrier integrity and regulation of inflammation in vitro using intestinal epithelial cell lines. Caco-2 or HT29 cell monolayers were challenged with enterotoxigenic (ETEC) or a ruminant isolate of Heidelberg in the presence or absence of one of six probiotic spp. strains. Among these, 506 excelled at exerting protective effects by significantly mitigating the decreased transepithelial electrical resistance (TEER) as assessed using area under the curve (AUC) ( < 0.0001) and increased apical-to-basolateral fluorescein isothiocyanate (FITC) dextran translocation ( < 0.0001) across Caco-2 cell monolayers caused by . Heidelberg or ETEC, respectively. Similarly, 506 and other probiotic strains significantly attenuated the . Heidelberg- and ETEC-induced increase in IL-8 from HT29 cells ( < 0.0001). Moreover, 506 significantly counteracted the TEER decrease ( < 0.0001) and FITC dextran translocation ( < 0.0001) upon challenge with . Finally, 506 significantly attenuated DON-induced TEER decrease ( < 0.01) and FITC dextran translocation ( < 0.05) and mitigated occludin and zona occludens (ZO)-1 redistribution in Caco-2 cells caused by the mycotoxin. Collectively, these results demonstrate the ability of 506 to confer protective effects on the intestinal epithelium in vitro upon challenge with enteric pathogens and DON known to be of particular concern in farm animals.