Assessment of possible biomedical applications of green synthesized TiONPs-an in-vitro approach.

Biomedical applications for various types of nanoparticles are emerging on a daily basis. Hence this research was performed to evaluate the antifungal (Aspergillus sp., Alternaria sp., Trichophyton sp., Candida sp., and Penicillium sp.), cytotoxicity (MCF10A cell lines), and antioxidant (DPPH) potential of Coleus aromaticus mediated and pre-characterized TiONPs were studied with respective standard methodology. Interestingly, the TiONPs exhibited significant antifungal activity on pathogenic fungal strains like Alternaria sp., Aspergillus sp. (31 ± 1.4), Penicillium sp. (31 ± 1.9) Trichophyton sp. (27 ± 2.1), and Candida sp. (26 ± 2.3) at high concentration (250 μg mL). However, the considerable levels of zone of inhibitions on fungal pathogens were recorded at 100 μg mL of TiONPs as well as it was considerably greater than positive control. It also demonstrated dose based anti-inflammatory and antidiabetic activities. The plant-mediated TiONPs demonstrated a maximum DPPH scavenging efficiency of 91% at a dosage of 250 μg mL, comparable to the positive control's 94%. Furthermore, TiONPs at 100 μg mL concentration did not cause cytotoxicity in MCF10A cell lines. At higher concentrations (250 μg mL), the nanoparticles showed the lowest cytotoxicity (17%). These findings suggest that C. aromaticus-mediated TiONPs have significant biomedical applications. However, in-vivo studies are needed to learn more about their (C. aromaticus-mediated TiONPs) potential biomedical applications.