sp. nov., isolated from sediment of mangrove plant , and comparative genomic analyses of the genus .

A Gram-negative, non-motile, strictly aerobic, rod-shaped bacterium, designated as H12, was isolated from the sediments of mangrove plant taken from Dapeng district, Shenzhen, PR China. The pairwise 16S rRNA gene sequence analysis showed that strain H12 shared high identity levels with species of the genus , with the highest similarity level of 98.5 % to SPO729, followed by 98.1 % to CN85. Phylogenetic analysis using core-genome sequences showed that strain H12 formed a cluster with type species of SPO729 and HB161719. The complete genome of strain H12 was 4 481 396 bp in size and its DNA G+C content was 56.7 mol%. The average nucleotide identity and digital DNA-DNA hybridization values among strain H12 and type species of genus were below the cut-off levels of 95-96 and 70 %, respectively. The predominant cellular fatty acids of strain H12 were iso-C (22.5 %) and C 7 (13.9 %). Ubiquinone-8 was detected as the major respiratory quinone. The polar lipids of strain H12 comprised one phosphatidylglycerol, one phosphatidylethanolamine, one unidentified aminoglycophospholipid, one unidentified glycophospholipid, three unidentified glycolipids, two unidentified aminolipids, and one unidentified lipid. Based on polyphasic evidence, strain H12 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H12 (=KCTC 92859=MCCC 1K08451). Comparative genomic analyses of strain H12 with strains of the genus reveal its potential in degradation of pectin.