Transcriptome reveals the role of the gene in mediating antibiotic resistance through cell envelope modulation in SCCF01.

HtpG, a bacterial homolog of the eukaryotic 90 kDa heat-shock protein (Hsp90), represents the simplest member of the heat shock protein family. While the significance of Hsp90 in fungal and cancer drug resistance has been confirmed, the role of HtpG in bacterial antibiotic resistance remains largely unexplored. This research aims to investigate the impact of the gene on antibiotic resistance in . Through the creation of gene deletion and complementation strains, we have uncovered the essential role of in regulating the structural integrity of the bacterial cell envelope. Our transcriptomics analysis demonstrates that the deletion of increases the sensitivity of to antimicrobial peptides, primarily due to upregulated lipopolysaccharide synthesis, reduced glycerophospholipid content, and weakened efflux pumps activity. Conversely, reduced sensitivity to β-lactam antibiotics in the Δ strain results from decreased peptidoglycan synthesis and dysregulated peptidoglycan recycling and regulation. Further exploration of specific pathway components is essential for a comprehensive understanding of -mediated resistance mechanisms, aiding in the development of antimicrobial agents. To our knowledge, this is the first effort to explore the relationship between and drug resistance in bacteria.