Background: possesses a cobalamin-dependent methionine synthase (MS). MS is putatively encoded by the PF3D7_1233700 gene, which is orthologous and syntenic in . However, its vulnerability as an antimalarial target has not been assessed.
Methods: We edited the PF3D7_1233700 and PF3D7_0417200 (dihydrofolate reductase-thymidylate synthase, DHFR-TS) genes and obtained transgenic parasites expressing epitope-tagged target proteins under the control of the ribozyme. Conditional loss-of-function mutants were obtained by treating transgenic parasites with glucosamine.
Results: DHFR-TS, but not MS mutants showed a significant proliferation defect over 96 h, suggesting that MS is not a vulnerable antimalarial target.
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| http://dx.doi.org/10.7717/peerj.16595 | DOI Listing |
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