Capillary electrophoresis separations with deep eutectic solvents as greener separation media: A proof-of-concept study.

Capillary electrophoresis (CE) has conventionally been classified into aqueous and non-aqueous categories based on the types of buffer solvents employed. Traditionally, non-aqueous CE has always been associated with the use of organic solvents, which are considered hazardous to health and environmentally detrimental. In this work, we introduce deep eutectic solvents (DESs) as CE separation media for the first time, presenting a novel and environmentally friendly approach to CE separations. The DES employed consists of proline and urea (Proline:Urea, PU), both of which are naturally occurring compounds that are readily available, cost-effective, and environmentally benign. Various fundamental aspects of the DES-type CE media were investigated, including thermal property, viscosity, electroconductivity, Joule heating effect, and compatibility with detectors. A simulated complex mixture of ten naphthalene-based compounds with varied charges and sizes was separated using the DES-based medium in capillary zone electrophoresis (CZE) mode. Moreover, we also established a DES-based micellar electrokinetic chromatography (MEKC) system utilizing Tween-20 as the surfactant. Six structurally similar naphthalene derivatives (isomers) that couldn't be resolved by CZE were effectively separated due to their strong hydrophobic interaction with Tween-20 micelles within the DES medium. Given that DESs are "designer" solvents with highly tunable properties and environmentally friendly characteristics, this study demonstrates the potential of employing DESs as an alternative to organic solvents for greener CE separations.