Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK; Center for Bioinformatics and Department of Computer Science, Saarland University, 66123 Saarbrücken, Germany. Electronic address:
Published: March 2024
AI Article Synopsis
Article Abstract
The isolation of sufficient amounts of intact nuclei is essential to obtain high-resolution maps of chromatin accessibility via assay for transposase-accessible chromatin using sequencing (ATAC-seq). Here, we present a protocol for tag-free isolation of nuclei from both cell walled and cell wall-deficient strains of the green model alga Chlamydomonas reinhardtii at a suitable quality for ATAC-seq. We describe steps for nuclei isolation, quantification, and downstream ATAC-seq. This protocol is optimized to shorten the time of isolation and quantification of nuclei.
Under ideal conditions, Chlamydomonas reinhardtii can photoacclimate to excess light through various short- and long-term mechanisms. However, how microalgae handle excess light stress once they exit exponential growth, and especially in stationary phase, is less understood. Our study explored C.
Diclofenac (DCF), a commonly used anti-inflammatory medication, presents environmental concerns due to its presence in water bodies, resistance to conventional wastewater treatment methods, and detection at increasing concentrations (ng/L to µg/L) that highlight DCF as a global emerging pollutant. While microalgae have been effective in degrading DCF in wastewater, immobilization into a matrix offers a promising approach to enhance treatment retention and efficiency. This study aimed to evaluate the efficacy of DCF removal using immobilized freshwater microalgae.
Chlamydomonas reinhardtii, a model green alga for expressing foreign proteins, faces challenges in multigene expression and enhancing protein expression level in the chloroplast. To address these challenges, we compared heterologous promoters, terminators and intercistronic expression elements (IEEs). We transformed Chlamydomonas chloroplast with a biolistic approach to introduce vectors containing the NanoLuc expression unit regulated by Chlamydomonas or tobacco promoters and terminators.
Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Suipacha 570, Rosario, Santa Fe S2002LRK, Argentina; Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario, Santa Fe S2002LRK, Argentina. Electronic address:
Chlamydomonas reinhardtii is a green alga that has been widely used as a model organism for studying various cellular processes, including starch metabolism. In this alga, starch undergoes continuous phosphorylation during its synthesis and degradation. We recently identified and characterized ChlreSEX4 (starch excess 4), a glucan phosphatase from C.
Department of Chemistry, University of Crete, Voutes Campus, Heraklion 70013, Greece; Environmental Chemical Processes Laboratory, University of Crete, Voutes Campus, Heraklion 70013, Greece. Electronic address:
As the concern for Ni contamination in the aquatic environment escalates, efforts for microalgal use in environmental monitoring and bioremediation are increasing. This study aims to evaluate the potential of Chlorella sorokiniana and Chlamydomonas reinhardtii for Ni bioremediation by investigating their physiological stress responses in Ni-contaminated environments. The analysis focuses on how Ni(II) uptake affects cell growth, nutrient metal homeostasis, and lipid unsaturation levels, as these parameters are critical indicators of metabolic stability and resilience essential for effective bioremediation.
