A fast and easy one-step purification strategy for plant-made antibodies using Protein A magnetic beads.

Front Plant Sci

ANGANY Innovation, 1 voie de l'innovation, Pharmaparc II, Val de Reuil, France.

Published: January 2024

A major difficulty to reach commercial- scale production for plant-made antibodies is the complexity and cost of their purification from plant extracts. Here, using Protein A magnetic beads, two monoclonal antibodies are purified in a one-step procedure directly from non-clarified crude plant extracts. This technique provides significant savings in terms of resources, operation time, and equipment.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10791999PMC
http://dx.doi.org/10.3389/fpls.2023.1276148DOI Listing

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  • In 2022, the monkeypox virus outbreak prompted the need for immediate public health responses, leading to the exploration of plant-derived monoclonal antibodies (mAbs) as potential treatments due to their antiviral properties.
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  • The findings confirm that the plant-made mAb, named H2, effectively binds to the MPXV EV and neutralizes it, marking a significant advancement in therapeutic strategies against monkeypox and highlighting the potential of plant-based antibodies for treating emerging infectious diseases.
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A major difficulty to reach commercial- scale production for plant-made antibodies is the complexity and cost of their purification from plant extracts. Here, using Protein A magnetic beads, two monoclonal antibodies are purified in a one-step procedure directly from non-clarified crude plant extracts. This technique provides significant savings in terms of resources, operation time, and equipment.

View Article and Find Full Text PDF

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