Plant Sci
School of Biological Science and Technology, University of Jinan, Jinan, Shandong, China. Electronic address:
Published: April 2024
Hornworts, as the sister group to liverworts and mosses, comprise bryophytes, which are critical in understanding the evolution of key land plant traits. Cinnamate 4-hydroxylase (C4H) catalyzes the second step of the phenylpropanoid pathway to synthesize the precursor of numerous phenolic compounds, such as lignin and flavonoids. However, C4H in the hornwort Anthoceros angustus has not yet been cloned and functionally characterized. In this work, we screened the transcriptome database of A. angustus and identified one C4H gene, AnanC4H. AnanC4H maintained conserved cytochrome P450 domains with other typical plant C4Hs. Ultraviolet B irradiation and exogenous application of methyl jasmonate (MeJA) induced the expression of AnanC4H to varying degrees. The coding sequence of AnanC4H was expressed in yeast, and the recombinant proteins were isolated. The recombinant proteins of AnanC4H catalyzed the conversion of trans-cinnamic acid to p-coumaric acid and catalyzed the conversion of 3-hydroxycinnamic acid to caffeic acid. AnanC4H showed higher affinity for trans-cinnamic acid than for 3-hydroxycinnamic acid, but there was no significant difference in the catalytic efficiency of AnanC4H for the two substrates in vitro. Moreover, the expression of AnanC4H in Arabidopsis thaliana led to an increase in both the lignin content and the number of lignified cells in stems. However, there was no significant change in flavonoid content in transgenic Arabidopsis plants.
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http://dx.doi.org/10.1016/j.plantsci.2024.111989 | DOI Listing |
Bioprocess Biosyst Eng
January 2025
Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Korea.
p-Coumaric acid (p-CA), an invaluable phytochemical, has novel bioactivities, including antiproliferative, anxiolytic, and neuroprotective effects, and is the main precursor of various flavonoids, such as caffeic acid, naringenin, and resveratrol. Herein, we report the engineering of Escherichia coli for de novo production of p-CA via the PAL-C4H pathway. As the base strain, we used the E.
View Article and Find Full Text PDFPlant Genome
March 2025
Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
The plant Polygonum capitatum (P. capitatum) contains a variety of flavonoids that are distributed differently among different parts. Nevertheless, differentially expressed genes (DEGs) associated with this heterogeneous distribution have not been identified.
View Article and Find Full Text PDFPlant Mol Biol
December 2024
Research Center of Crop Stresses Resistance Technologies/ MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River, Yangtze University, Jingzhou, 434025, China.
Anthocyanin regulation can be fruitfully explored from a diverse perspective by studying distantly related model organisms. Land plants pioneers faced a huge evolutionary leap, involving substantial physiological and genetic changes. Anthocyanins have evolved alongside these changes, becoming versatile compounds capable of mitigating terrestrial challenges such as drought, salinity, extreme temperatures and high radiation.
View Article and Find Full Text PDFHeliyon
November 2024
Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran.
Plant Physiol Biochem
November 2024
College of Food Science and Technology, Nanjing Agricultural University, Nanjing, 210095, China. Electronic address:
After harvesting, Gynura bicolor DC (G. bicolor) undergoes rapid quality deterioration, including decay, nutrient loss, and reactive oxygen species (ROS) burst, greatly limiting its shelf life. This study was performed to evaluate the effects of treatment with sucrose and 1-methylcyclopropene (1-MCP) on indices of quality deterioration, ROS metabolism, and phenylpropanoid metabolism, with the goals of resisting oxidative stress and improving the postharvest quality of G.
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