Histones catalyze DNA strand incision at apurinic/apyrimidinic (AP) sites accompanied by the formation of reversible but long-lived DNA-protein cross-links at 3'-termini (3'-histone-DPCs). However, the chemical structures of 3'-histone-DPCs are not well characterized, and whether they are formed in cells is uncertain. In this study, we developed a liquid chromatography with tandem mass spectrometry workflow to characterize DPCs produced from the reaction of histones with AP sites and wish to report evidence that histones cross-link to incised AP sites via Schiff bases. We also demonstrated for the first time that 3'-histone-DPCs are produced endogenously in human embryonic kidney 293T cells.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.chemrestox.3c00377 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Chemical characterization of Eucalyptus (Eucalyptus globulus) leaf essential oil and evaluation of its antifungal, antibacterial and antioxidant activities.
Cell Mol Biol (Noisy-le-grand)
January 2025
Laboratory of Cellular Toxicology, Faculty of Science, Department of Biology, Badji Mokhtar University, Annaba, Algeria.
Article Synopsis
- The study analyzes essential oil extracted from dry Eucalyptus globulus leaves, focusing on its chemical composition and potential health benefits.
- The oil consists of 20 identified compounds, primarily linalool, and exhibits antifungal properties against Fusarium roseum and antibacterial effects against Pseudomonas savastanoi.
- While it shows moderate antioxidant activity, it differs from typical eucalyptus oils by being a linalool chemotype rather than eucalyptol-dominant.
An investigation of antiproliferative and antioxidant properties of crude extracts from Sedum nicaeense All. (Crassulaceae).
Pak J Pharm Sci
January 2025
Department of Pharmacy, Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman, Jordan.
This study evaluated the antioxidant and antiproliferative effects of aqueous, ethanolic and methanolic extracts of Sedum nicaeense flowers and leaves. The MTT assay assessed cytotoxicity against colorectal cancer cells (Caco-2, HCT-116), breast cancer cells (T47D, MCF-7) and normal fibroblasts (MRC-5), while the ferric-reducing antioxidant power (FRAP) assay measured antioxidant capacity. Essential oils from flowers and leaves were analyzed using gas chromatography-mass spectrometry (GC-MS).
View Article and Find Full Text PDFAnalysis of Chemical Components of Penicillium chrysogenum Crude Extract Using Liquid Chromatography Coupled With Q-Exactive Orbitrap Mass Spectrometry Combined With GNPS Molecular Networking.
Rapid Commun Mass Spectrom
April 2025
School of Pharmaceutical Science and Technology, Faculty of Medicine, Tianjin University, Tianjin, Tianjin, China.
A Chinese isolate of the fungus Penicillium chrysogenum was analyzed using liquid chromatography coupled with Q-Exactive Orbitrap mass spectrometry combined with Global Natural Products Social Networking (GNPS) on culture condition leading to the rapid identification of 20 secondary metabolites. Among them are eight polyketones, two phthalides, six diketopiperazine alkaloids, and others. A meleagrine network was examined and proposed as a promising candidate for new natural products.
View Article and Find Full Text PDFMetabolomic Analysis of Patients With Patent Foramen Ovale and Migraine Using Liquid Chromatography-Mass Spectrometry Technology.
Rapid Commun Mass Spectrom
March 2025
Department of Cardiology, Xinjiang Traditional Chinese Medicine Hospital, Xinjiang, China.
Article Synopsis
- This study investigates how metabolic profiles change in patients with patent foramen ovale (PFO) and migraines before and after surgery, using metabolomics techniques.
- Significant differences in metabolites like linoleic acid and quinolinic acid were observed after surgery, indicating potential diagnostic markers for these patients.
- The research highlights the importance of metabolic pathways related to inflammation and oxidative stress in understanding migraines associated with PFO.
Multiclass Steroid Profiling in Short-Finned Pilot Whale Blubber Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
Rapid Commun Mass Spectrom
March 2025
Chemical Sciences Division, National Institute of Standards and Technology, Charleston, South Carolina, USA.
Rationale: Wildlife scientists are quantifying steroid hormones in a growing number of tissues and employing novel methods that must undergo validation before application. This study tested the accuracy and precision of liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods for use on blubber samples from short-finned pilot whales (Globicephala macrorhynchus). We expanded upon a method for corticosteroid quantification by adding analytes and optimizing internal standard (IS) application.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!