AI Article Synopsis
- The study investigates the role of long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) in the development of coronary artery disease (CAD), highlighting the importance of their interactions.
- Using data from CAD patients and controls, 534 differentially expressed genes (DEGs) were identified, with specific miRNAs linked to these DEGs, notably hsa-miR-92a-3p, which targeted the most genes.
- CDKN2B-AS1 was found to regulate hsa-miR-92a-3p, suggesting that this lncRNA may act as a sponge for the miRNA, indicating a potential new regulatory mechanism in CAD involving the CDKN2B-AS1
Article Abstract
Background: LncRNAs, miRNAs, and the sponge effect between them exert diverse biological influences on the pathogenesis and progression of coronary artery disease (CAD), thus necessitating an exploration of the lncRNA-miRNA-gene regulatory network in CAD.
Methods: Expression profile GSE98583 was obtained from NCBI, containing the data of 12 CAD patients and 6 controls. Limma package was utilized to determine the differentially expressed genes (DEGs). Functional enrichment analysis was performed by DAVID. The CAD-related miRNA-DEG associations were retrieved via HMDD and miRTarBase, and the CAD-related lncRNA-miRNA associations were retrieved via LncRNADisease and starBase. The CAD-related lncRNA-miRNA-DEG regulatory network was constructed by combining these associations. The dual luciferase test was carried out to validate the connections among lncRNA, miRNA, and gene.
Results: Overall, 534 DEGs were identified between CAD samples and controls, including 243 up-regulated and 291 down-regulated, and were enriched in various gene ontology biological processes and KEGG pathways. The CAD-related miRNAs targeting DEGs included hsa-miR-206, has-miR-320b, has-miR-4513, has-miR-765, and has-miR-92a-3p, and hsa-miR-92a-3p regulated the most DEGs. In the lncRNA-miRNA associations, only CDKN2B-AS1 regulated the CAD-related miRNA, hsa-miR-92a-3p, which was validated using the dual luciferase test.
Conclusions: CDKN2B-AS1 may act as an hsa-miR-92a-3p sponge to regulate the downstream DEGs in CAD. CDKN2B-AS1/ hsa-miR-92a-3p/GATA2 might be a novel mechanism for CAD.
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| http://dx.doi.org/10.23736/S2724-5683.23.06441-4 | DOI Listing |
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