AI Article Synopsis
- * Using single-cell RNA sequencing, researchers explored the development of germinal center (GC) B cells after immunization and identified three distinct cell populations in the GC light zone (LZ).
- * One LZ population showed early signs of differentiating into PCs but had affinity levels similar to those remaining in the GC, indicating that the process of starting PC development might not depend on antibody affinity.
Article Abstract
Long-lived plasma cells (PCs) secrete antibodies that can provide sustained immunity against infection. High-affinity cells are proposed to preferentially select into this compartment, potentiating the immune response. We used single-cell RNA-seq to track the germinal center (GC) development of Igh B cells, specific for the Plasmodium falciparum circumsporozoite protein (PfCSP). Following immunization with Plasmodium sporozoites, we identified 3 populations of cells in the GC light zone (LZ). One LZ population expressed a gene signature associated with the initiation of PC differentiation and readily formed PCs in vitro. The estimated affinity of these pre-PC B cells was indistinguishable from that of LZ cells that remained in the GC. This remained true when high- or low-avidity recombinant PfCSP proteins were used as immunogens. These findings suggest that the initiation of PC development occurs via an affinity-independent process.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10922795 | PMC |
| http://dx.doi.org/10.1016/j.immuni.2023.12.010 | DOI Listing |
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