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| http://dx.doi.org/10.1161/JAHA.123.030825 | DOI Listing |
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Comparative Evaluation of Open-Source Bioinformatics Pipelines for Full-Length Viral Genome Assembly.
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Institute of Biology, ELTE Eötvös Loránd University, 1117 Budapest, Hungary.
The increasingly widespread application of next-generation sequencing (NGS) in clinical diagnostics and epidemiological research has generated a demand for robust, fast, automated, and user-friendly bioinformatics workflows. To guide the choice of tools for the assembly of full-length viral genomes from NGS datasets, we assessed the performance and applicability of four open-source bioinformatics pipelines (shiver-for which we created a user-friendly Dockerized version, referred to as dshiver; SmaltAlign; viral-ngs; and V-pipe) using both simulated and real-world HIV-1 paired-end short-read datasets and default settings. All four pipelines produced consensus genome assemblies with high quality metrics (genome fraction recovery, mismatch and indel rates, variant calling F1 scores) when the reference sequence used for assembly had high similarity to the analyzed sample.
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Organ Support and Automation Technologies Group, U.S. Army Institute of Surgical Research, JBSA Fort Sam Houston, San Antonio, TX 78234, USA.
Prehospital medical care is a major challenge for both civilian and military situations as resources are limited, yet critical triage and treatment decisions must be rapidly made. Prehospital medicine is further complicated during mass casualty situations or remote applications that require more extensive medical treatments to be monitored. It is anticipated on the future battlefield where air superiority will be contested that prolonged field care will extend to as much 72 h in a prehospital environment.
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Xuejiawan Power Supply Company, Ordos 010300, China.
Recently, massive intelligent applications have emerged for the smart grid (SG), such as inspection and sensing. To support these applications, there have been high requirements on wireless communication for the SG, especially in remote areas. To tackle these challenges, a UAV-assisted heterogeneous wireless network is proposed in this paper for the SG, where multiple UAVs and a macro base station collaboratively provide a wide range of communication services.
View Article and Find Full Text PDFDesign and Evaluation of a Cloud Computing System for Real-Time Measurements in Polarization-Independent Long-Range DAS Based on Coherent Detection.
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Institute of Mechanical Intelligence, Scuola Superiore Sant'Anna, Via G. Moruzzi 1, 56124 Pisa, Italy.
CloudSim is a versatile simulation framework for modeling cloud infrastructure components that supports customizable and extensible application provisioning strategies, allowing for the simulation of cloud services. On the other hand, Distributed Acoustic Sensing (DAS) is a ubiquitous technique used for measuring vibrations over an extended region. Data handling in DAS remains an open issue, as many applications need continuous monitoring of a volume of samples whose storage and processing in real time require high-capacity memory and computing resources.
View Article and Find Full Text PDFBinary Transformer Based on the Alignment and Correction of Distribution.
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Faculty of Information Engineering and Automation, Kunming University of Science and Technology, Kunming 650500, China.
Transformer is a powerful model widely used in artificial intelligence applications. It contains complex structures and has extremely high computational requirements that are not suitable for embedded intelligent sensors with limited computational resources. The binary quantization technology takes up less memory space and has a faster calculation speed; however, it is seldom studied for the lightweight transformer.
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