Creating in vitro culture platforms for monkey embryos is crucial for understanding the initial 4 weeks of early primate embryogenesis. Here, we present a protocol to culture cynomolgus monkey embryos in vitro for 25 days post-fertilization and to delineate the key developmental events of gastrulation and early organogenesis. We describe steps for culturing with a 3D system, immunofluorescence analysis, single-cell RNA sequencing, and bioinformatic analysis. For complete details on the use and execution of this protocol, please refer to Gong et al. (2023)..
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10826423 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2023.102835 | DOI Listing |
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